Influence of Dietary Protease on Growth Performance and Carcass Yield of Indian River Meat Broiler Chickens



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Submitted Aug 25, 2022
Published Nov 28, 2022
10.24018/ejfood.2022.4.6.563

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The aim of the present study was to evaluate the effect of a multi-component dietary protease complex on the growth performance and carcass yields of commercial broiler chickens. A total of 630 Indian River broiler DOCs were considered and assigned to five dietary treatments namely: 1) Positive control (PC), a standard diet with no supplemental protease, 2) Negative control (NC), a diet similar to PC but reduced in CP, digestible EAA, and ME based on supplier’s nutrient matrix recommendation, 3) PC+125 ppm protease (PC+125), 4) NC+125 ppm protease (NC+125), and 5) NC+200 ppm protease (NC+200). The birds were fed experimental diets for 28 days. Body weight (BW), body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR), and survival rate were recorded weekly. On day 28, three birds per treatment were sacrificed for carcass yield analysis. FI was similar among the treatment groups except in the 2nd week where broilers fed PC+125, NC+125, and NC+200 diets had significantly higher (P<0.05) FI than those fed the PC diet. In terms of BW and BWG, there were no differences among the treatment groups at 1st and 2nd weeks, however, at 3rd and 4th weeks, broilers fed the PC+125, NC+125, and NC+200 diets were significantly heavier (P<0.05) than those fed the PC and NC diets. A similar trend was also observed in FCR. Among the carcass yield parameters, the percentage weights of thigh, breast, and heart in birds fed the NC+200 diet were significantly increased (P<0.05) as compared to those fed the NC diet, while results for PC, PC+125, and NC+125 were intermediate between the two treatment groups. Overall, the results of present study demonstrated that the supplementation of protease complex either on a standard or reduced diet may improve the overall production performance of commercial broiler chickens and offers benefits on certain aspects of carcass yield.

Keywords: broiler chickens, carcass yield, growth performance, protease.

References

  1. Khattak FM, Pasha TN, Hayat Z, Mahmud A. Enzymes in poultry nutrition. J. Anim. Plant Sci. 2006;16(1-2):1-7. http://thejaps.org.pk/docs/16_1-2_2006/Khattak.pdf.
     Google Scholar
  2. Applegate TJ, Angel R. Protein and amino acid requirements for poultry. Anim Sci. 2008 https://s3.wp.wsu.edu/uploads/sites/346/2014/11/Protein-and-amino-acid-for-poultry-final.pdf.
     Google Scholar
  3. Angel CR, Saylor W, Vieira SL, Ward N. Effects of a mono-component protease on performance and protein utilization in 7-to 22-day-old broiler chickens. Poult. Sci. 2011;90(10):2281-6.
     Google Scholar
  4. Doskovic V, Bogosavljevic-Boskovic S, Pavlovski Z, Milosevic B, Skrbic Z, Rakonjac S, et al. Enzyme in broiler diets with special reference to protease. World Poult. Sci. J. 2013;69(2):343-360. https://doi.org/10.1017/S0043933913000342
     Google Scholar
  5. Simbaya J, Slominski BA, Guenter W, Morgan A, Campbell LD. The effects of protease and carbohydrase supplementation on the nutritive value of canola meal for poultry. Anim. Feed Sci. Tech. 1996;61(1-4):219-234. https://doi.org/10.1016/0377-8401(95)00939-6.
     Google Scholar
  6. Yu B, Wu ST, Liu CC, Gauthier R, Chiou PW. Effects of enzyme inclusion in a maize-soybean diet on broiler performance. Anim. Feed Sci. Tech. 2007;134(3-4):283-294. https://doi.org/10.1016/j.anifeedsci.2006.09.017.
     Google Scholar
  7. Freitas DM, Vieira SL, Angel CR, Favero A, Maiorka A. Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease. J. Appl. Poult. Res. 2011;20(3):322-334. https://doi.org/10.3382/japr.2010-00295.
     Google Scholar
  8. Fru-Nji F, Kluenter AM, Fischer M, Pontoppidan K. A feed serine protease improves broiler performance and increases protein and energy digestibility. J. Poult. Sci. 2011;48(4):239- 246. https://doi.org/10.2141/jpsa.011035.
     Google Scholar
  9. Kamel NF, Ragaa M, El-Banna RA, Mohamed FF. Effects of a mono-component protease on performance parameters and protein digestibility in broiler chickens. Agric. Agric’l Sci. Proce. 2015; 6:216-225. https://doi.org/10.1016/j.aaspro.2015.08.062.
     Google Scholar
  10. Liu SY, Selle PH, Court SG, Cowieson AJ. Protease supplementation of sorghum-based diets enhances amino acid digestibility coefficients in four small intestinal sites and accelerates their rates of digestion. Anim. Feed Sci. Tech. 2013;183(3-4):175-183. https://doi.org/10.1016/j.anifeedsci.2013.05.006.
     Google Scholar
  11. Mohammadigheisar M, Kim IH. Addition of a protease to low crude protein density diets of broiler chickens. J. Appl. Anim. Res. 2018 Jan 1;46(1):1377-1381. https://doi.org/10.1080/09712119.2018.1512862.
     Google Scholar
  12. Silva J.M, de Oliveira NR, Gouveia AB, Vieira RA, dos Santos RO, Minafra CS, et al. Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler diets. Czech J. Anim. Sci. 2021 Jan 1;66(1):29-37. https://doi.org/10.17221/134/2020-CJAS.
     Google Scholar
  13. Gitoee A, Janmohammadi H, Taghizadeh A, Rafat SA. Effects of a multi-enzyme on performance and carcass characteristics of broiler chickens fed corn-soybean meal basal diets with different metabolizable energy levels. J. Appl. Anim. Res. 2015 Jul 3;43(3):295-302. http://dx.doi.org/10.1080/09712119.2014.963103.
     Google Scholar
  14. Kalmendal R, Tauson R. Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet. Poult. Sci. 2012 Jun 1;91: 1387-1393. https://doi.org/10.3382/ps.2011-02064.
     Google Scholar
  15. Cowieson AJ, Zaefarian F, Knap I, Ravindran V. Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health. Anim. Prod. Sci. 2016;57(6): 1058-1068. https://doi.org/10.1071/AN15740.
     Google Scholar
  16. Cardinal KM, Moraes ML, Andretta I, Schirmann GD, Belote BL, Barrios MA, et al. Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease. R. Bras. Zootec. 2019;481-11. https://doi.org/10.1590/rbz4820180232.
     Google Scholar
  17. Anderson LN, Christgau S, Dalboge H, Dambmann C, Kauppinen MS, Kofod LV, et al. An enzyme with protease activity; 2001. US Pat. No. 408: 257-290.
     Google Scholar
  18. Smith A. Protease reduces environmental impact of broiler production. Feed Enzymes. Anim. Nutr. Health, DSM; 2015. https://www.poultryworld.net/health-nutrition/protease-reduces-environmental-impact-of-broiler-production/.
     Google Scholar
  19. Indian River Broiler Nutrition Specifications. Aviagen Group; 2019. Available from: https://ap.aviagen.com/assets/Tech_Center/LIR_Broiler/IRBroilerNutritionSpecs2019-EN.pdf.
     Google Scholar
  20. Bates J. AOAC. Official Methods of Analysis, Association of Official Analytical Chemists. Virginia, USA; 1994.
     Google Scholar
  21. Twining SS. Fluorescein Isothiocyanate-Labeled Casein Assay for Proteolytic Enzymes. Anal. Biochem. 1984;143(1): 30-34. https://doi.org/10.1016/0003-2697(84)90553-0.
     Google Scholar
  22. IBM SPSS: Statistical Computer Package Program 20.00. https://www.ibm.com/analytics/spss-statistics-software.
     Google Scholar
  23. Ghazi S, Rooke JA, Galbraith H, Morgan A. Effect of adding protease and alpha- galactosidase enzymes to soybean meal on nitrogen retention and true metabolizable energy in broilers. Brit. Poult. Sci. 1997; 38: 28. https://agris.fao.org/agris-search/search.do?recordID=GB1997044118.
     Google Scholar
  24. Yan F, Garribay L, Arce J, Lopez-Coello C, Camacho D, Disbennet P, et al. Effects of protease supplementation on broiler performance and in vitro protein digestibility. Proc. Aust. Poult. Sci. Symp., pp.134-137. Sydney, New South Wales, Australia; 2012. https://www.cabdirect.org/cabdirect/abstract/20123169102.
     Google Scholar
  25. Espino TM, Sapin AB, Tambalo RD, Unida FB. Acid protease from Monascus species Biotech 3064 and other microbial enzymes and feed additives in broiler diets. Proc. Ann. Conv. Phil. Soc. Micro., pp.121-133. Philippines; 2000. https://agris.fao.org/agris-search/search.do?recordID=PH2002001320.
     Google Scholar
  26. Ghazi SR, Rooke JA, Galbraith H, Bedford MR. The potential for the improvement of the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels. Brit. Poult. Sci. 2002;43(1):70-77. https://doi.org/10.1080/00071660120109935.
     Google Scholar
  27. Ghazi S, Rooke JA, Galbraith H. Improvement in the nutritive value of soybean meal by protease and alpha-galactosidase treatment in broiler cockerels and broiler chicks. Brit. Poult. Sci. 2003; 44:410-418. https://doi.org/10.1080/00071660310001598283.
     Google Scholar
  28. Yadav JL, Sah RA. Supplementation of corn-soybean based broiler's diets with different levels of acid protease. J. Ins. Agric. Anim. Sci. 2005 Apr 1; 26:65-70. https://doi.org/10.3126/jiaas.v26i0.613.
     Google Scholar
  29. Al-juboori J. Effect of protease supplementation in broiler feed on growth performance, carcass yield and total nitrogen retention in fecal matter and litter. Electronic Theses and Dissertations, 142, Stephen F Austin State University 2017. https://scholarworks.sfasu.edu/etds/142.
     Google Scholar
  30. Hartman R. Wheat-based diets improved by enzymes. J. Appl. Poult. Res. 1996;5: 167-172.
     Google Scholar


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