The study was carried out to assess some microbiological qualities of smoked African catfish, Clarias gariepinus retailed in Sango and Ota markets in Ogun state, Nigeria. Six smoked fish retailers were randomly selected from the markets and 36 fish samples were collected. The samples of smoked catfish were assessed weekly for a period of six weeks for microbiological parameters such as Total plate count (TPC), Total coliforms count (TCC), Staphylococcus count and mould count. In this study, the microbial load of the smoked Clarias gariepinus samples retailed in the two study sites increased weekly during the period of storage. TPC increased from 4.75±0.014 log10 CFU/g for smoked samples from Ota market, retailer 1 (OMR1) in week 0 to 5.11 ±0.021 log10 CFU/g for samples from Ota market, retailer 3 (OMR3) in week 5. TCC ranged from 1.47±0.001 log10 CFU/g to 2.43±0.049 log10 CFU/g; Staphylococcus from 2.15±0.070 log10 (CFU/g) to 2.66±0.014 log10 (CFU/g) and 1.82 ±0.070 log10 CFU/g to 2.92±0.071 log10 CFU/g for mould counts. The smoked Clarias gariepinus from the markets could not keep well till the 6th week. Hence, it can be inferred that smoked catfish with moisture content of about 10% or less will have a shelf life of about 4 - 5 weeks under ambient storage conditions before the effect of spoilage microorganisms.
Olatunde, A. A. Approaches to the study of fisheries biology in Nigerian inland waters. Proc. nat. con. of two decades of research on lake kainji. Ayeni and Olatunde (eds). pp. 1538-1541, 1989.
Ayeloja, A. A, George, F. O. A, Jimoh, W. A, Shittu, M. O, and Abdulsalami, S. A. Microbial load on smoked fish commonly traded in Ibadan, Oyo State. Nigeria. J. Appl. Sci. Environ. Manage, vol. 22, no. 4, pp. 493– 497, 2018.
Adesehinwa, A. O. K, Ayanda, J. O, and Bolorunduro, P. I. Adoption of improved fish preservation technologies in Northwestern Nigeria. Tropicultural, vol. 23, pp. 117-123, 2005.
Olaleye, O. N, and Abegunde, T. A. Microbiological safety assessment of selected smoked fish in Lagos metropolis. British Microbiology Research Journal, vol. 9, no. 3, pp. 1-5, 2015
Aberoumand, A. Estimation of microbiological variations in minced lean fish products. World J. Fish and Marine Sci., vol. 2, no. 3, pp. 204–207, 2010.
Clucas, I. J, and Ward, I. J. Post–harvest fisheries development: a guide to handling/preservation, processing and quality. Natural Resource Institute Chatham Meantime, Kent, United Kingdom, pp. 443, 1996.
Asiedu, M, and Sanni, A. I. Chemical composition and microbiological changes during spontaneous and starter culture fermentation of Enam Ne-Setaakye, a West African fermented fish-carbohydrate product. Euro. Food Res. Tech., vol. 215, no. 1, pp. 8-12, 2002.
Bako, W. S. The role of women in fish processing: Handling and marketing in Kainji Lake basin. Proceedings of 19th Annual Conference of the Fisheries Society of Nigeria (FISON), Nov.29-Dec.3, 2004, Ilorin, Nigeria, 2005.
Basti, A. A, Misaghi, A, Salehi, T. Z, and Kamkar, A. Bacterial pathogens in fresh, smoked and salted Iranian fish. Food Control, vol. 17, pp. 183-188, 2006.
Jakhar, J. K, Anirudh Kumar, A, and Vardia, H. K. Hygenicity and nutritional quality of traditional dried and smoked fishes at kawardha fish market, (Chhattisgarh), India. The Bioscan, vol. 10, no. 3, pp. 1099-1102, 2015.
Heintz, M. L, and Johnson, J. M. The Incidence of Listeria spp., Salmonella spp. and Clostridium botulinum in smoked fish and shellfish. Journal of Food Protection, vol. 61, no. 3, 318-323, 1998.
Fawole, M. O, and Osho, B. A. Laboratory manual of microbiology. Shalom prints Ibadan, Nigeria. pp. 25–30, 1995.
International Commission on Microbiological Specifications for Foods-(ICMSF). Microorganisms in Foods 2, sampling for microbiological analysis. Principles and Specific Applications, 2nd edn. Oxford: Blackwell Science, 1986.
Akinwumi, F. O, and Adegbehingbe, K. T. Microbiological analysis of three of smoked fish obtained from the Ondo State, Nigeria. Food and Public Health, vol. 5, no. 4, pp. 122-126, 2015.
Surendran, P, Nirmala, Thampuran, K, Narayanannambiar, V, and Lalitha, K. V. Manual on microbiological examination of seafood. Laboratory M, and Mathew, P. T. (Eds), CIFT and SOFT, Cochin, 2nd edn. pp. 28-45, 2006.
Varnam, A. H. Foodborne pathogens: an illustrated text. Mosby- Year Book, Inc., London, 1991.
Omojowo, F. S, and Ihuahi, J. A. Microbiological quality and safety of smoked fish from kainji lake area. African Scientist, vol. 7, no. 4, 2006.
Ibrahim, B. U, Baba, J, and Sheshi, M. S. Isolation and identification of bacteria associated with fresh and smoked fish (Clarias gariepinus) in Minna Metropolis, Niger State. Nigeria. Journal of Applied and Environmental Microbiology, vol. 2, no. 3, pp. 81-85, 2014.
Abolagba, O. J, Adekunle, A. T, Dede, A. P. O, and Omoigui, G. O. Microbial assessment of smoked fish (Clarias spp) in Benin Metropolis, Edo State, Nigeria. Nigerian Journal of Agriculture, Food and Environment, vol. 7 no. 3, pp. 55-58, 2011.
Abolagba, O. J, and Uwagbai, E. C. A. Comparative analysis of the microbial load of smoke dried fishes (Ethmalosa fimbriata and Pseudolithus elongatus) sold in Oba and Koko markets in Edo and Delta States, Nigeria at different seasons. Australian Journal of Basic and Applied Sciences, vol. 5, no. 5, pp. 544-550, 2011.
Martin, A. M. Fisheries processing biochemical applications. Chapman and Hall Publisher, London, 1994.
Josephson, D. B, Lindsay, R. C, and Stuiber, D. A. Influence of processing on the volatile compounds characterizing the flavor of pickled fish. Journal of Food Science, vol. 52, no. 1, pp. 10–14, 1987.
Ibeh, I. N, Uriah, N, Ogonor, J. I. Dietary exposure to aflatoxin in Benin city, Nigeria: a possible public health concern. Int. J. Food Microbiol. Vol. 14, pp. 171-174, 1991.
Mitchell T. G. Medical Mycology In: Jawetz, Melnick and Adelberg’s (eds). Medical Microbiology 24th Edition. McGraw Hill USA. pp. 621-625, 2007.
Gibson, A. M, Baranyi, J, Pitt, M. J, Eyles, M. J, and Roberts, T. A. Predicting fungal growth: The effect of water activity on Aspergillus flavus and related species. Int. J. Food Microbial. Vol. 23, pp. 419-431, 1994.
Ibanga, I. J, Moses, E. A, Edet, E. J, and Moses, A. E. Microbial and some heavy metals analysis of smoked fishes sold in urban and rural markets in Akwa Ibom State, Nigeria. Calabar J. Health Sci 3(2):73-79, 2020
Olayemi, F.F., Raji, A.O. and Adedayo, M.R. Microbiological quality of catfish (Clarias gariepinus) smoked with Nigerian Stored Products Research Institute (NSPRI) developed smoking kiln. Int. Res. J. Microbiol. Vol. 3, pp. 426-430, 2012.
Daramola, J. A, Fasakin, E. A, and Adeparusi, E. O. Changes in physicochemical and sensory characteristics of smoke-dried fish species stored at ambient temperature. African Journal of Food Agriculture Nutrition and Development, vol. 7, no. 6, pp. 157-160, 2007.
Omojowo, F. S, Omojasola, P. F, Kolawole, M. O, Ngwu, E. O, Oluborode, G. B, Adetunji, C. O. Effect of brining on the microbial quality and safety of smoked catfish. New York Science Journal, vol. 3, no. 6, 2010.