Use of Simple Sequence Repeat Marker to Assess the Segregating Population of Rice (Oryza sativa) in field for Grain Length Quality Parameter

##plugins.themes.bootstrap3.article.main##

  •   Sanjay U. Borale

  •   Shailesh D. Kumbhar

Abstract

Rice grain quality is of paramount importance in the consumer preference for rice varieties and therefore the development of rice varieties should match to the consumer preference. Assessment of segregating population of breeding material in rice for quality parameter made it possible by the use of simple sequence repeat(SSR) marker. The F2 mapping population of a cross between a long slender rice genotype VDN 1137-1-1 and a short slender rice landrace Badshabhog was studied for the inheritance of grain length and to identify the marker associated with grain type. The F2 population showed 3:1 ration for long slender to short slender grain type. This segregating population was assessed by 24 SSR markers using bulk segregant analysis. Among these 24 SSR Markers, a marker RM 147 reported polymorphism between the long slender and short slender parents and respective bulks. Genetic analysis indicated goodness of fit for single gene model indicating the association of RM147 with grain length gene in the segregating population. Therefore, SSR marker RM 147 is of immense value in the assessment of grain quality parameter in rice during the development of rice varieties.


Keywords: Rice, Grain Length quality, SSR marker, Segregating population, Marker Association

References

Gadal,N., Shrestha, J., Poudel,M.N and Pokhrel, B. 2019. A review on production status and growing environment of rice in Nepal and in the world. Archives of Agriculture and Environmental Science. 4(1): 83-87.

Rao, S. A., Khan, M. A., Mc Nailly, T. and Khan, A. A., 1997. Cause and effect relationships of yield and yield components in rice (Oryza sativa L.). J. Genet. Breed, 15(1): 1-5.

Calingacion, M., Laborte, A., Nelson, A., Resurreccion, A., Concepcion, J. C., Daygon, V. D., Mumm, R., Reinke, R., Dipti, S., Bassinello, P. Z., Manful, J., Sophany, S., Lara, K. C., Bao, J. S., Xie, L. H., Loaiza, K., El-hissewy, A., Gayin, J., Sharma, N., Rajeswari, S., Manonmani, S., Rani, N. S., Kota, S., Indrasari, S. D., Habibi, F., Hosseini, M., Tavasoli, F., Suzuki, K.,Umemoto, T., Boualaphanh, C., Lee, H. H., Hung, Y. P., Ramli, A., Aung, P. P., Ahmad, R., Wattoo, J. I., Bandonill, E., Romero, M., Brites, C. M., Hafeel, R., Lur, H. S., Cheaupun, K., Jongdee, S., Blanco, P., Bryant, R., Lang, N. T., Hall, R. D., Fitzgerald, M., 2014 Diversity of global rice markets and the science required for consumer-targeted rice breeding. PLoS One 9: e85106. https://doi.org/10.1371/journal.pone.0085106.

Doyle, J.J., and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissues. Focus. 12: 13-15.

Michelmore, W. R., Paran, I and Kesseli,R.V. 1991. Identification of marker linked to diseases resistance genes by bulked segregants analysis, a rapid method to detect the markers in specific genetic region by using the segregating population. Proc. Natl. Acad. Sci. USA 88: 9828–9832.

Huang, Ning., Parco, A., Mew, T., Magpantay, G., McCouch, Susan., Guiderdoni, E., Xu, J., Subudhi, P., Angeles, E. R. and Khush G. S., 1997. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown plant hopper resistance in a doubled haploid rice population. Molecular Breeding.3:105–113.

Pal, Surender., Jain, S., Saini, N. Aarti and Jain, R. K., 2004. Identification of microsatellite markers for differentiating some Basmati and non- Basmati rice varieties. J. of Biotechnology.vol.3: 519-526.

Li, Jiming., Jinhua, X., Silvana, G., Longying, Jiang., Wan, Y., Qiyun, D., Longping, Y., and McCouch, S. R. 2004. QTL detection for rice grain quality traits using an interspecific backcross population derived from cultivated Asian (O. sativaL.) and African (O. glaberrimaS.) rice. Genome 47: 697–704.

Wang, X. Y., Wan, J. M., Jiang, L., Wang, J. K., Zhai, H. Q., Weng, J. F., Wang, H. L., Lei, C. L., Wang, J. L., Zhang, X., Cheng, Z. J. and Guo, X. P., 2006. QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects. Theor. Appl. Genet. 112: 1258–1270.

Li, Mao-mao., Xu, lei., Ren, Jun-fang., Cao, Gui-lan., Yunli-qin., He, Hao-hua., Han, Long zhi and Ko, h. 2010. Identification of quantitative trait loci for grain traits in japonica rice. Agricultural Sciences in China. 9(7): 929-936.

Singh, Yogendra and Singh, U.S. 2012.Simple Sequence Repeats markers and grain quality characteristics for genetic divergence and selective identification of Aromatic rices. International J. Adv. Biotec.& Res. 3: 711-719.

Chen, X., Temnykh, S., Xu, Y., Cho, Y. G., McCouch, S. R. 1997. Development of microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor. Appl. Genet. 95: 553–567.

Lang, N.T., Khush, G.S., Huang, N. and Bau, B.C., 2001. Fine mapping for blast resistance gene in rice (Oryza sativa L.) using bulked segregrant analysis. Omon rice 9: 1-8.

Zhou, J. H., Wang, J. L., Xu, J. C., Lei, C. L. and Ling, Z. Z., 2004. Identification and mapping of rice blast resistance gene Pi-g(t) in the cultivar Guangchangzhan. Plant Pathol. 53 (2): 191-196.

Lee, S., Wamishe, Y., Jia, Y and Liu,G. 2009. Identification of two major resistance genes against race IE-1k of Magnaporthe oryzae in the indica rice cultivar Zhe733. Mol. Breeding. 24: 127-134.

Ashkani, A., Rafii M.Y., Sariah M., Siti, Nor Akmar M., Rusli I., Abdul, R A. and Latif, M. A., 2011. Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). Gen. Mol. Res. 10(3): 1345-1355.

Pal Surender., Jain, S., Saini, N. Aarti., and Jain, R.K. 2004. Identification of microsatellite markers for differentiating some Basmati and non Basmati rice varieties. J. of Biotechnology. Vol.3: 519-526.

Jiang, G.H., Xue-Y. H., Cai-Guo, X. U., Xiang-Hua L. I. and Yu-Qing, H.E. 2005. Identification of quantitative trait loci for grain appearance and milling quality Using a doubled-haploid rice Population. J. Integrative Pl. Bio. (Formerly Acta Botanica Sinica), 47(11): 1391-1403.

Bai, Xu feng., Luo, L. , Yan, W. , Kovi, Mallikarjuna R., Zhan, W. and Xing, Y. 2010. Genetic dissection of rice grain shape using a recombinant inbred line population derived from two contrasting parents and fine mapping a pleiotropic quantitative trait locus qGL7. BMC Genet.11:16 (doi: 10.1186/1471-2156-11-16).

Rabiei, B., Valizadeh, M., Ghareyazie, B., Moghaddam, M. and Ali, A. J.2004. Identification of QTLs for rice grain size and shape of Iranian cultivars using SSR markers. Euphytica. 137: 325–332.

Wan, X.Y., Wan, J.M., Weng, J.F., Jiang, L., Bi, J.C., Wang, C.M., Zhai, H.Q., 2005. Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theoretical and Applied Genetics .110: 1334–1346.

##plugins.themes.bootstrap3.article.details##

How to Cite
Borale, S. U., & Kumbhar, S. D. (2021). Use of Simple Sequence Repeat Marker to Assess the Segregating Population of Rice (Oryza sativa) in field for Grain Length Quality Parameter. European Journal of Agriculture and Food Sciences, 3(1), 132-135. https://doi.org/10.24018/ejfood.2021.3.1.239