Effect of Diatomaceous Earth on Growth Rate, Egg Production, Feed Conversion Efficiency and Parasitic Load in Hens Raised on Deep Litter


  •   Robert Alex Isabirye

  •   Savino Biryomumaisho

  •   James Okwee-Acai

  •   Samuel Okello

  •   George William Nasinyama


The efficacy of diatomaceous earth (DE) on growth rate, egg production and on increasing feed conversion efficiency in deep litter raised layer hens was evaluated. The study was conducted at Mukono Zonal Agricultural Research and Development Institute (MUZARDI) in Uganda. Worms were collected from fresh intestines of indigenous chicken obtained from Kalerwe chicken market near Kampala. The DE was mined in Pakwach (formerly Nebbi) district in Northern Uganda. Chickens of the Lohmann Brown breed raised on deep litter were studied. At 7 weeks the birds were divided into 5 treatment groups, A, B, C, D and E each composed of 40 birds. Groups C, D and E were given an oral dose containing 250 embryonated eggs of A. galli while groups A and B were not infected. The chicks were weighed; and subjected to feeding trials as arranged below: A – Non-infected birds on DE (4%) supplemented diet; B –non-infected birds on neither piperazine (a conventional de-wormer) nor DE; C – infected birds on DE supplemented diet; D – infected birds on piperazine; and group E – infected birds on neither DE nor piperazine applied. Fecal samples were collected and analysed in the laboratory biweekly at week 16 till week 22 respectively for fecal egg counts. In a subsequent experiment, day-old layer chicks from Lohmann Brown strain but different from those used in earlier experiments, were used to assess the effect of DE on egg production. At 17 weeks of age the 420 were divided as follows: 6 treatment groups each having 7 replicates and each replicate having 10 birds. This study showed that DE can be used successfully in growing pullet diets to correct nutritional mineral imbalance since it supplies more than 14 trace elements and other elements. Diatomaceous earth also enables pullets to cope with Ascaridia galli load; and 2% DE supplementation did not have significant improvement on egg production as compared to no supplementation at all. However, reduction in egg production was experienced when supplementation with levels of DE higher that 2% was applied. Results from biweekly fecal analyses showed significant differences in fecal egg counts (p<0.05); and treatment by group (p<0.05).

Keywords: Diatomaceous earth, Ascaridia galli, feed conversion, laying hens, Uganda



NDP 2015, National Development Plan 2015/16 - 2019/20

UBOS (2018), Uganda Bureau of Statistics (2018) Statistical Abstracts.

Berg C, (2001). Health and Welfare in organic poultry production. ACTa Vet Scand. Suppl. 95:37 – 45.

Bejaei M. and Cheng K. M. (2010). Effects of demographic characteristics and attitudes of consumers on table egg consumption. Poult.Sci:. 89) Suppl. 1): 644 – 645.

Bunch T. R., Bond C., Buhl, K., Stone D. (2013). Diatomaceous Earth General Fact Sheet; National Pesticide Information Center, Oregon State University Extension Services. http://npic.orst.edu/factsheets/degen.html. (Accessed August 23, 2019).

Adeyemo G. O. (2010). “Effects of Exposure Duration to Cottonseed Cake-Based Diets on Broiler Performance,” International Journal of Poultry Science, Vol. 9, No. 2, 2010, pp. 162-166. doi:10.3923/ijps.2010.162.166

Fields P.G. (2000). Diatomaceous earth: Advantages and limitations. In: Proc. VII International Working Conference on Stored-Product Protection. Sichuan Publishing House of Science and Technology, Chengdu, China 1:781–784.

Adebiyi A.O., Sokunbi, A.O., & Ewuola, E.O. (2009). Performance evaluation and bone characteristics of growing cockerels fed diets containing different levels of diatomaceous earth. Middle-East J. Sci. Res. 4 (1): 36-39.

John O. (2006). Alternative to antibiotics in poultry production. Nutritional Biotechnology in the feed and food industries. T.P. Lyons U.K. pp 57-67.

Hendel B. (2009). “Water and salt”. Laboratory Science, London 45-50.

Zhang, Q.; Shu, X.; Guo, X.; Mo, D.;Wei, S.; Yang, C. (2016). Effect of ions on sorption of tylosin on clay minerals. RSC Adv. 6, 53175–53181.

Subramanyam B., and Roesli R. (2000). Inert dusts. In: Subramanyam, B., Hagstrum, D.W. (Eds.), Alternatives to Pesticides in Stored-product IPM. Kluwer Academic Publishers, Dordrecht, pp 321 – 380.

Adedeji, O. S., Amao, S. R., Oguntunde, M. M., & Dada, I. D. 2014. Evaluation of general performance and carcass qualities of organically raised broiler chickens from day old to 12 weeks of age. IJAIR. 2:1473- 2319.

Sadeghi, A. A., Safaei, A., & Aminafshar, M. 2014. The effect of dietary oil sources on performance, serum corticosterone level, antibody titers and IFN-y gene expression in broiler chickens. Kafkas. Univ. Vet. Fak. Derg. 20: 857-862.

Bennett DC, Yee A, Rhee YJ, and Cheng KM (2011). Effect of diatomaceous earth on parasite load, egg production, and egg quality of free-range organic laying hens. Poultry Science 90:1416–1426 doi: 10.3382/ps.2010-01256

Höglund, J. and Jansson, D.S. (2011). Infection dynamics of Ascaridia galli in non-caged laying hens. Veterinary Parasitology 180:267– 273.

Cox DD and Todd AC (1962). Survey of gastrointestinal parasitism in Wisconsin dairy cattle. J. Am.Vet. Med Assoc. 141:706-709.

Cox WR, Lemiski D (1989). Prevalence of gastrointestinal nematodes in dairy heifers in Western Canada. Can Vet. J. 30:666-668.

Soulsby EJL (1982). Helminths, Arthropods and Protozoa of Domestic Animals. 7thed Bailliere Tindall, London, UK.,

Foreyt WJ (2001). Veterinary Parasitology: Reference Manual. 5th ed. Blackwell Publishing, Ames, IA

Zajac, AM and Conboy GA (2006). Veterinary Clinical Parasitology 7th Ed. Blackwell Publishing, Ames, IA.

Skrivan, M., Englmaierová, M., Skrivanová, E. & Bubancová, I. (2015). Increase in lutein and zeaxanthin content in the eggs of hens fed marigold flower extract. Czech Journal of Animal Science. 60:89–96.

A.O.A.C. (2016). Official Methods of Analysis of AOAC INTERNATIONAL (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD, USA, Official Method 2008.01

Isabirye, R.A., Waiswa, C., Kabi, F., Nanyeenya, W.N., Biryomumaisho, S., Acai-Okwee, J., Okello, S., Akello B. O., Mwesigwa, M., Lumu R., and Nasinyama G. W. (2019b). Efficacy of diatomaceous earth on Ascaridia galli, blood parameters and on ectoparasites in chicken. J. of Agric. Sc and Food Technol.5 (9) pp. 205-217

North M O (1984). Breeder Management. In Commercial Chicken Production Manual. The Avi Publishing Company Inc. Westport Connecticut. pp 298 – 321.

Tello-Velamazán J, Newbold J, Edwards H and Pachebat J (2015). Use of diatomaceous earth as a dietary supplement in organic hens and its effects on parasite load, egg production and egg quality. WSPA conference. Malaga Spain: 205-213.

Reid W.M. and Carmon J.L. (1958). Effects of numbers of Ascaridia galli in depressing weight gains in chicks. Journal of Parasitology 44: 183-186.

Isabirye, R.A., Waiswa, C., Kabi, F., Nanyeenya, W.N., Biryomumaisho, S., Acai-Okwee, J., Okello, S., Akello B. O., Mwesigwa, M., Lumu R., and Nasinyama G. W. (20191). Diatomaceous earth usage in the agriculture sector in Uganda and its characterization: Current status and anticipated developments. J. of Agric. Sc. and Food Technol. 5(4)-pp 87–95.

Quisenberry, J.H (1967). The use of a clay in poultry feed. Clays & Clay Miner., 16:267 – 270.

Mallet, S., Delord, P., Juin, H and Lessire, M. 2005. Effect of in feed talc supplementation on broiler performance. Animal Research, EDP Sciences, 54: 485-492.

Janet J. D. (2009). “A few notes on safe solutions”. Inc. fossil shell flour (Diatomaceous Earth). Eur. Med. PP 211-215.

Pym, R., & Alders, R. (2016). Chapter 22. Helping smallholders to improve poultry production. In: Achieving sustainable production of poultry meat. (pp. 441–471). Cambridge, UK: Burleigh Dodds Science Publishing

Bernard G., Worku M., &Ahmedna M. (2009). The effect of diatomaceous earth on parasite infected goats. Bull. Georg. Natl. Acad. Sci. 3: 130-134.

Kiaei, S. M. M., Modirsanei, M., Farkhoy, M., &Taghdiri A. 2002. Effects of diatomite and natural zeolite supplementation on the performance of broiler chicks and litter moisture. J. Fac. Vet. Med, Univ of Tehran. 57:19-27.

Tran, S. T., Bowman, M. E., & Smith, T. K. (2015). Effects of a silica-based feed supplement on performance, health, and litter quality of growing turkeys. Poult. Sci. 94(8):1902.


How to Cite
Isabirye, R. A., Biryomumaisho, S., Okwee-Acai, J., Okello, S., & Nasinyama, G. W. (2021). Effect of Diatomaceous Earth on Growth Rate, Egg Production, Feed Conversion Efficiency and Parasitic Load in Hens Raised on Deep Litter. European Journal of Agriculture and Food Sciences, 3(1), 97-103. https://doi.org/10.24018/ejfood.2021.3.1.207

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